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Anti-GLUT3 (extracellular) Antibody (#AGT-023) is a highly specific antibody directed against an epitope of the rat Glucose Transporter 3 protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from rat and mouse samples. It will not recognize GLUT3 from human samples.
Anti-GLUT3 (extracellular)-FITC Antibody (#AGT-023-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.
Immunogen
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Peptide (C)KDFLNYTLEERLED, corresponding to amino acid residues 39 - 52 of rat Glucose Transporter 3 (Accession Q07647). Extracellular, 1st loop.
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HomologyMouse – identical.
RRIDAB_2756645.
PurityAffinity purified on immobilized antigen.
FormLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
IsotypeRabbit IgG.
SpecificityWill not recognize human GLUT3.
LabelFluorescein isothiocyanate (FITC).
Standard quality control of each lotWestern blot analysis (unlabeled antibody, #AGT-023), and direct flow cytometry (labeled antibody).
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution50 µl double distilled water (DDW).
Antibody concentration after reconstitution1 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).