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ANR-176 Anti-RAGE (extracellular) Antibody

发布日期:2021-01-20 11:23   浏览量:
Cat #: ANR-176
Host: Rabbit
Type: Polyclonal
Form: Lyophilized

Anti-RAGE (extracellular) Antibody (#ANR-176) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize RAGE from human, rat, and mouse samples. This antibody will recognize both the membrane-bound and the soluble form of the RAGE receptor.

Applications: IFC, WB
May also work in: ICC, IHC, IP, LCI
Reactivity: H, M, R

Application key:

 CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

 H- Human, M- Mouse, R- Rat
 
For research purposes only, not for human use

Applications

 
Western blot
  • Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
    Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
    1, 2. Anti-RAGE (extracellular) Antibody (#ANR-176), (1:600).
    3, 4. Anti-RAGE (extracellular) Antibody, preincubated with RAGE (extracellular) Blocking Peptide (#BLP-NR176).
  • Western blot analysis of human PANC-1 pancreatic carcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 7) and mouse BV-2 microglia cell line lysate (lanes 4 and 8):
    Western blot analysis of human PANC-1 pancreatic carcinoma cell line lysate (lanes 1 and 5), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 3 and 7) and mouse BV-2 microglia cell line lysate (lanes 4 and 8):
    1-4. Anti-RAGE (extracellular) Antibody (#ANR-176), (1:600).
    5-8. Anti-RAGE (extracellular) Antibody, preincubated with RAGE (extracellular) Blocking Peptide (#BLP-NR176).
Indirect flow cytometry
  • Cell surface detection of RAGE in live intact mouse BV-2 microglia cell line:
    Cell surface detection of RAGE in live intact mouse BV-2 microglia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-RAGE (extracellular) Antibody (#ANR-176), (2.5 µg) + goat-anti-rabbit-FITC.

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