AER-019 Anti-EphA2 (extracellular) Antibody

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):

1, 2. Anti-EphA2 (extracellular) Antibody (#AER-019), (1:200).
3, 4. Anti-EphA2 (extracellular) Antibody, preincubated with EphA2 (extracellular) Blocking Peptide (#BLP-ER019).

Western blot analysis of human U87-MG glioblastoma cell line lysate (lanes 1 and 5), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 6), human LNCaP prostate adenocarcinoma cell line lysate (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 4 and 8):

1-4. Anti-EphA2 (extracellular) Antibody (#AER-019), (1:200).
5-8. Anti-EphA2 (extracellular) Antibody, preincubated with EphA2 (extracellular) Blocking Peptide (#BLP-ER019).

Expression of EphA2 in rat hippocampus.

Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA2 (extracellular) Antibody (#AER-019), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region, showed immunoreactivity (green) in neuronal profiles in both soma (vertical arrows) and apical dendrites of pyramidal neurons (horizontal arrows). B. Pre-incubation of the antibody with EphA2 (extracellular) Blocking Peptide (#BLP-ER019), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.

Expression of EphA2 in mouse cortex.

Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-EphA2 (extracellular) Antibody (#AER-019), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. EphA2 immunoreactivity (green) appeared in neuronal profiles in both soma (vertical arrows) and apical dendrites of pyramidal neurons (horizontal arrows). B. Pre-incubation of the antibody with EphA2 (extracellular) Blocking Peptide (#BLP-ER019), suppressed staining. Cell nuclei are stained with DAPI (blue).

Cell surface detection of EphA2 in live intact human THP-1 monocytic leukemia cells:

___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-EphA2 (extracellular) Antibody (#AER-019), (2.5 µg) + goat-anti-rabbit-FITC.