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AGR-051 Anti-GPR35 (extracellular) Antibody

发布日期：2021-01-19 10:49   浏览量：
Cat #: AGR-051
Host: Rabbit
Type: Polyclonal
Form: Lyophilized

Anti-GPR35 (extracellular) Antibody (#AGR-051) is a highly specific antibody directed against an extracellular epitope of the mouse GPR35. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR35 from mouse and rat samples. The antibody will not recognize the receptor from human samples.

 

Applications: IFC, IHC, LCI, WB
May also work in: CBE, ICC, IP
Reactivity: M, R

Application key:

 CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

 H- Human, M- Mouse, R- Rat
 
For research purposes only, not for human use

Applications

 
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-GPR35 (extracellular) Antibody (#AGR-051), (1:200). 3-4. Anti-GPR35 (extracellular) Antibody, preincubated with GPR35 (extracellular) Blocking Peptide (BLP-GR051).
  • Western blot analysis of rat dorsal root ganglion lysate:
    Western blot analysis of rat dorsal root ganglion lysate:
    1. Anti-GPR35 (extracellular) Antibody (#AGR-051), (1:200). 2. Anti-GPR35 (extracellular) Antibody, preincubated with GPR35 (extracellular) Blocking Peptide (BLP-GR051).
  • Western blot analysis of rat small intestine lysate:
    Western blot analysis of rat small intestine lysate:
    1. Anti-GPR35 (extracellular) Antibody (#AGR-051), (1:200). 2. Anti-GPR35 (extracellular) Antibody, preincubated with GPR35 (extracellular) Blocking Peptide (BLP-GR051).
Immunohistochemistry
  • Expression of GPR35 in rat dorsal root ganglion.
    Expression of GPR35 in rat dorsal root ganglion.
    Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-GPR35 (extracellular) Antibody (#AGR-051), followed by goat anti-rabbit-AlexaFluor-488. A. GPR35 immunoreactivity (green) appeared in neuron profiles (arrows). B. Pre-incubation of the antibody with GPR35 (extracellular) Blocking Peptide (#BLP-GR051), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of GPR35 by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
    Cell surface detection of GPR35 by indirect flow cytometry in live intact mouse BV-2 microglia cell line:
    ___ Cells.
    ___ Cells + Goat-anti-rabbit-FITC.
    ___ Cells + Anti-GPR35 (extracellular) Antibody (#AGR-051), (5µg) + goat-anti-rabbit-FITC.

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